A simple, precise, accurate and reproducible spectrophotometric method has been developed for simultaneous estimation of Naproxen and Esomeprazole Magnesium trihydrate by employing first order derivative zero crossing method in methanol. The first order derivative absorption at 318 nm (zero cross point of Naproxen) was used for quantification of Esomeprazole Magnesium trihydrate and 276 nm (zero cross point of Esomeprazole Magnesium trihydrate) for quantification of Naproxen. The linearity was established over the concentration range of 1-11 μg/ml and 10-35 μg/ml for Esomeprazole Magnesium trihydrate and Naproxen with correlation coefficient r 2 0.999 and 0.999, respectively. The mean % recoveries were found to be in the range of 98.88 – 99.33 % and 99.11 – 99.55 % for Esomeprazole Magnesium trihydrate and Naproxen, respectively. The proposed method has been validated as per ICH guidelines and successfully applied to the estimation of Esomeprazole Magnesium trihydrate and Naproxen in their combined Tablet dosage form.
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